Research Paper Volume 15, Issue 9 pp 3230—3248

Figure 3. Reporter gene activity under the control of the 5’ regulatory region of Hes 1, Oct4a and Cyclin D1 in Msi 1 knockdown BCCs. (A) BCCs, knockdown for Msi 1 or transfected with vector were studied for reporter gene in which luciferase was downstream of the Hes 1 5’ regulatory region. Normalized luciferase levels are expressed as fold changes over pGL3 transfectants. The data are presented as the mean±SD of four independent experiments, each in duplicate. * p<0.05 vs vector alone. (B) The studies in `B’ are repeated except for the reporter gene containing Oct4a 5’ regulatory region. REST siRNA served as a positive control. The data are presented as for `A’. Each experiment was performed in duplicate. * p<0.05 vs vector alone. (C) The studies in `B’ are repeated except for analyses for cyclin D1 activity and the data for three independent experiments with each performed in duplicate, presented as for `A’. * p<0.05 vs vector alone.