Research Paper Volume 15, Issue 14 pp 6894—6904

Figure 5. Dephosphorylation by GSK3β enhanced MCL-1 reduction. (A) AGS and BCG-823 cells were transfected with siNC or siCIP2A for 48 h followed by celastrol (5 μM) for 6 h, lysed, and the lysates were probed with indicated antibodies. (B) AGS and BCG-823 cells were transfected with siNC or siGSK3β for 48 h, lysed, and the lysates was probed with anti-MCL-1 antibody. (C) AGS and BCG-823 cells were transfected with siNC or siCIP2A for 48 h, followed by celastrol (5 μM) for 6 h, lysed, the expression of PP2A, GSK-3β, pGSK-3β and MCL-1 were detected by Western blot assay.