Research Paper Volume 15, Issue 16 pp 8275—8297
Integrative analysis links autophagy to intrauterine adhesion and establishes autophagy-related circRNA-miRNA-mRNA regulatory network
Figure 10. The effect of hub ARCs on cell autophagy, myofibroblast transformation and collagen deposition. The mRNA expression of Beclin1 (A) LC3B (B) α-SMA (C) FN1 (D) Col III (E) was detected through RT-PCR. The percentage of α-SMA positive cell was calculated in immunofluorescence results (F). The phosphorylation level of Beclin1 (G) protein expression of LC3B (H) and p62 (I) and release of Col III (J) and FN1 (K) was evaluated through ELISA. The immunofluorescence images of α-SMA in ESCs (L). *p <0.05, compared with Control; **p <0.05, compared with TGF-β+siRNA-NC.