Research Paper Volume 15, Issue 20 pp 11114—11130

Figure 5. Upregulating NKD1 alleviates PAH symptoms in MCT-induced mouse PAH model. (A) The relative protein level of NKD1 in dissected PAs of MCT-treated mice was assessed by western blotting (post hoc for LSD test; n = 8 samples). RVSP (B) and RVHI (C) of MCT-treated mice were measured (post hoc for LSD test; n = 7 samples). RVSP indicates right ventricular systolic pressure. RVHI indicates right ventricular hypertrophy index. (D) Representative H-E staining pictures of lung tissues in vehicle- or MCT-treated mice after transfection of Ad-Con or Ad-Nkd1 were shown. The black arrows indicate peripheral PAs of lung tissues. Bar = 50 μm. Data were shown as mean ± S.D. ^**P < 0.01 and ^***P < 0.001 denoted statistical comparison between the two marked groups, respectively.