Research Paper Volume 15, Issue 20 pp 11227—11243

Figure 6. Inhibition of the MAPK/ERK/CREB pathway in the CA1 region reduces the protective effect of EA against postoperative cognitive dysfunction. (A) The CA1 was bilaterally injected with AAV-shCREB 21 days before surgery. (B) ZsGreen was specifically expressed in hippocampal CA1 region. Scale bar = 50 μm. (C) Traveling trajectory in the open field. (D–F) Total distance traveled, time in the central zone and traveled distance in the central zone of the open field in the three groups. (G) Exploration time to the familiar and novel objects during the novel object recognition phase. (H) Recognition index. (I–M) Swimming trajectory, escape latency, number of crossings of the platform area, total time spent in the target quadrant and average swimming speed in the MWM test. Data are shown as means ± standard deviations. ^*p < 0.05, ^**p < 0.01.