Research Paper Volume 15, Issue 24 pp 14764—14790
Conjoint research of WGCNA, single-cell transcriptome and structural biology reveals the potential targets of IDD development and treatment and JAK3 involvement
Figure 3. (A) tSNE visualization of all cells in NP tissues after sample integration, 9 clustered were generated. (B) Dot plot showing the NP cells marker genes ACAN, SOX9 and MIA within tSNE map; and violin plot showing the expression of macrophage marker genes CD14 and MRC1. (C) tSNE visualization of all cells in NP tissues between degeneration and non-degeneration group. (D) Selection of the most variable genes for monocle analysis. (E) PCA dimensional reduction by monocle algorithm between degeneration and non-degeneration group. (F) Volcano scatter plot of the genes analyzed by monocle method. (G) Hierarchical clustering heatmap of the analyzed DEGs in each cell.