Research Paper Volume 16, Issue 3 pp 2141—2160
Moxibustion prevents tripterygium glycoside-induced oligoasthenoteratozoospermia in rats via reduced oxidative stress and modulation of the Nrf2/HO-1 signaling pathway
Figure 8. Regulatory effect of DM on the Nrf2/HO-1 signaling pathway in rats with TGS-induced OAT. (A) IHC staining of Nrf2 and HO-1 in testicular tissues (×400; scale bar: 50 μm). (B, C) MOD values of Nfr2 and HO-1 (n = 6). (D) Western blotting analysis of the indicated proteins in each group. (E, F) Quantitative analysis of Nfr2 and HO-1 expression from Western blots (n = 3). (G, H) mRNA expression of Nrf2 and HO-1 in testicular tissues (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001.