Research Paper Volume 16, Issue 3 pp 2736—2752

Figure 1. Identification of circ_0039787 in CC. (A, B) Differentially expressed circRNAs in CC tissues and paracancerous tissues in the GSE113696 and GSE102686 datasets. (C) Flow chart for screening differentially expressed circRNAs. (D) circ_0039787 mRNA levels in CC and paracancerous tissues, N = 56. (E) circ_0039787 expression levels in serum samples from healthy controls and CC patients, N = 40. (F) The ROC analysis for detection of CC patients from health controls using circ_0039787. (G) The mRNA levels of circ_0039787 were compared between CC and control cell lines. (H) Sanger sequencing revealed that circ_0039787 was generated by reverse-splicing exons 4, 5, and 6 of the parent gene C16orf70. (I) The mRNA levels of circ_0039787 and C16orf70 were measured after treatment with RNase R. (J) The alterations in mRNA levels of circ_0039787 and C16orf70 were observed at different time points following the addition of actinomycin D. (K) The mRNA levels of circ_0039787 were measured in both the nuclei and cytoplasm. ^*P < 0.05, ^**P< 0.01, ^***P < 0.001.