Research Paper Volume 15, Issue 14 pp 7187—7218
Identification of TGF-β-related genes in cardiac hypertrophy and heart failure based on single cell RNA sequencing
Figure 10. MYC transcriptionally regulates ADAMTS2. (A) Three predicted binding sites of the MYC protein in the ADAMTS2 promoter region are shown. (B) Boxplot showing the expression of MYC mRNA in GSE141910. * P < 0.05. (C, D) Co-transfection of the mutant ADAMTS2 promoter recombinant vector and the MYC expression vector in 293 cells is verified using dual luciferase reporter gene assays. * P < 0.05 versus the oe-NC group. (E) Binding of MYC to the ADAMTS2 promoters is tested using ChIP assays. * P < 0.05 versus the IgG antibody group.