Research Paper Volume 15, Issue 19 pp 10057—10071

Figure 5. The LINC00667-miR-143-3p-ZEB1-associated ceRNA regulatory network. (A, B) The binding sites between LINC00667 and miR-143-3p and between miR-143-3p and ZEB1 searched through the ENCORI database (starbase.sysu.edu.cn). (C, D) The influence of miR-143-3p mimics on LINC00667-Wt, LINC00667-Mut, ZEB1-Wt, and ZEB1-Mut luciferase activities were checked using a dual-luciferase reporter assay. (E) The impact of biotin-labeled miR-143-3p on LINC00667 monitored by RNA Pull-down assay. (F) A498 cells were transfected with miR-NC, miR-143-3p, and miR-143-3p+LINC00667. The miR-143-3p level was gauged via qRT-PCR. LINC00667 overexpression plasmids and miR-143-3p mimics were employed to transfect Caki, while LINC00667-in and miR-143-3p-in were harnessed to transfect A498. (G, H) The mRNA level of ZEB1 in Caki and A498 was assayed using qRT-PCR. (I–L) The ZEB1 level in Caki and A498 cells was assessed by WB. **P<0.01, ***P<0.001 (vs. miR-NC/NC/NC-in). ^^P<0.01 (vs. miR-143-3p). N=3.