Research Paper Volume 15, Issue 20 pp 11471—11488
The Wnt/β-catenin signaling pathway inhibits osteoporosis by regulating the expression of TERT: an in vivo and in vitro study
Figure 3. Validation of the effect of TERT on osteogenic differentiation and apoptosis in BMSCs. (A, B) The siRNA-mediated depletion of TERT assessed by western blotting. (C–E) The specific proteins related to osteogenesis differentiation detected by Western blotting, including OPN and Runx2. (F) ALP staining in NC group and TERT-depleted BMSCs. (G) ALP activity detected by ALP activity assay kit in NC group and TERT-depleted BMSCs. (H, I) The Hoechst 33342/PI staining and quantification results. (J–L) The specific proteins related to apoptosis detected by Western blotting, including Bcl-2 and BAX. All results are performed as mean ± SD. #P < 0.05, ##P < 0.01 vs. NC group. n = 6.