Research Paper Volume 15, Issue 23 pp 13680—13692

Figure 5. TXNIP is a direct target of miR-135a-5p. (A) Schematic of miR-135a-5p binding site in the 3′UTR of TXNIP mRNA and corresponding mutation. (B) Free energy score predicted by the RNAhybrid database. (C) The luciferase reporter plasmids (TXNIP-WT and TXNIP-Mut) were co-transfected into 293T cells with miR-135a-5p mimic or mimic control for 24 h, followed by measurement of luciferase activity. (D, E) Human NP cells were transfected with miR-135a-5p mimic/inhibitor or their negative controls for 24 h. (D) The qRT-PCR analysis of miR-135a-5p expression. (E) Detection of TXNIP expression using qRT-PCR and western blot. Data represent the mean ± SD from three independent experiments. ***P < 0.001.