Research Paper Volume 15, Issue 24 pp 14749—14763
Downregulating miR-184 relieves calcium oxalate crystal-mediated renal cell damage via activating the Rap1 signaling pathway
Figure 4. IGF1R was targeted by miR-184. The rat renal calcinosis model was induced through the intraperitoneal transfusion of glyoxylate (80 mg/kg/d; 200 μL). (A) qRT-PCR was used to ascertain IGF1R expression in rat kidney tissues. ***P<0.001 (vs. Normal). N=10. (B) Pearson analysis evaluated the correlation between IGF1R and miR-184 in the renal calcinosis model. (C) The complementary base sequence of IGF1R and miR-184 in the Starbase. (D–G) Dual luciferase and RIP experiments validated the binding correlation between miR-184 and IGF1R. nsP>0.05, **P<0.01, ***P<0.001 (vs. miR-NC). N=3.