Research Paper Volume 15, Issue 24 pp 14591—14606

Figure 3. SRSF7 knockdown-mediated senescence is accompanied by the generation of MDM2 splice variants. (A, B) HDFs (DT2) were transfected with siRNA against either negative control (NC) or SRSF7 for 4 days. (A) mRNA level of SRSF7, TP53, MDM2 using qPCR. (^**p < 0.01 vs. NC by student t-test). (B) Western blot analysis of MDM2 splice variants, p53 and p21. (C) mRNA level of MDM2 and TP53 in dose-dependent OSIS using qPCR. (^**p < 0.01 vs. Con by student t-test). (D) Western blot analysis of MDM2 splice variants, p53 and p21 in dose dependent OSIS, along with western blot analysis of MDM2 knockdown. HDFs (DT2) were transfected with siRNA against either NC or MDM2. (E) mRNA level of MDM2 and TP53 in RS using qPCR. (^*p < 0.05 and ^**p < 0.01 vs. DT2 by student t-test). (F) Western blot analysis of MDM2 splice variants, p53 and p21 in RS.